School of Chemical Engineering and Materials Science
Recombinant Protein Solubility Prediction
Type (or cut and paste) your protein sequence below, click on the "Submit" button, and the solubility probability of your protein will be calculated. The statistical model predicts protein solubility assuming the protein is being overexpressed in Escherichia coli. If there are numbers, spaces, or other characters in your sequence, don't worry, they won't affect the calculation. For more information on the solubility model used here, see the references below.
References:
R.G. Harrison. 2000. Expression of soluble heterologous proteins via fusion with NusA protein. inNovations. 11:4-7. PDF file
Davis, G.D., Elisee, C., Newham, D.M. and R.G. Harrison. 1999. New fusion protein systems designed to give soluble expression in Escherichia coli. Biotechnol. Bioeng. 65(4):382-8. PubMed Abstract
Wilkinson, D.L. and R.G. Harrison. 1991. Predicting the solubility of recombinant proteins in Escherichia coli. Bio/Technology. 9: 443-448. PubMed Abstract
Please send questions or comments to rharrison@ou.edu.
